Complete bisulfite conversion of GC-rich DNA in less than 3 hours.

A coupled heat denaturation/conversion reaction step steamlines the conversion of non-methylated cytosines into uracil.

Desulfonation and recovery of bisulfite-treated DNA with a spin column.

Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

Format	Spin Column
DNA Recovery	> 75%
Processing Time	3 hrs
Equipment	Microcentrifuge
Binding Capacity	5 µg/prep
Elution Volume	≥ 10 µl
Conversion Efficiency	> 99%
Input	Plasmid, genomic, and endonuclease digested DNAs.