点击图片查看原图
539480 Proteinase K, Tritirachium album ( Proteinase K, Tritirachium album )
Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
|
产品信息
|
|
|
Form
|
White lyophilized solid
|
|
Formulation
|
Lyophilized from calcium acetate
|
|
Molar mass
|
28500
|
|
Specific activity
|
≥40 units/mg protein
|
|
Unit definition
|
One unit is defined as the amount of enzyme that will liberate 1.0 µmol tyrosine from casein per min at 37°C, pH 7.5.
|
|
Activity
|
≥30 units/mg dry weight
|
|
Contaminants
|
DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C
|
|
pH value
|
optimum 7.5 - 12.0
|
|
Solubility
|
50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0
|
|
EC number
|
3.4.21 .14
|
|
CAS number
|
39450-01-6
|
|
Store and ship information
|
|
|
Storage
|
+2°C to +8°C
|
|
Ship
|
Ambient Temperature Only
Irritant |
|
安全标示
|
|
|
S Phrase
|
S: 22-24-26-36/37
Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
|
R Phrase
|
R: 36/37/38-42
May cause sensitization by inhalation. |